Framework for qPCR modeling and analysis of low copy number sample

Published:November 10, 2022DOI:


      For the diagnosis of infectious diseases and cancer risk estimation, the importance of accurate quantification of DNA or RNA templates in a sample has been increased. The author utilized the Cq distribution calculation method based on binomial distribution and observed that the distribution is multi-modal, indicating that the distribution cannot be represented by unimodal, such as Log-Normal or Gamma distributions. By combining the result of this model with the Poisson distribution, the Cq values probability distribution for a dilution experiment can be calculate as multi-modal distribution.


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