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Nuclear DNA SNP profiles derived from human hair shaft

Published:October 26, 2022DOI:https://doi.org/10.1016/j.fsigss.2022.10.080

      Abstract

      DNA from rootless hair shaft is fragmented and in low abundance. We have developed methods using capture hybridization and massively parallel sequencing to generate nuclear genotype profiles from hair. Using a panel targeting 215 SNP loci highly informative for ancestry and identity, our results elicited a probability of inclusion of approximately one in greater than 9.6E+39. This shows the potential of this approach to obtain nuclear genotype information from hair shaft DNA.

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      References

        • Opel K.L.
        • Fleishaker E.L.
        • Nicklas J.A.
        • Buel E.
        • McCord B.R.
        Evaluation and quantification of nuclear DNA from human telogen hairs.
        J. Forensic Sci. 2008; 53: 853-857
        • Gutierrez R.
        • LaRue B.
        • Houston R.
        Novel extraction chemistry and alternative amplification strategies for use with rootless hair shafts.
        J. Forensic Sci. 2021; 66: 1929-1936
        • Court D.S.
        Mitochondrial DNA in forensic use.
        Emerg. Top. Life Sci. 2021; 5: 415
        • Ávila-Arcos M.C.
        • Cappellini E.
        • Romero-Navarro J.A.
        • et al.
        Application and comparison of large-scale solution-based DNA capture-enrichment methods on ancient DNA.
        Sci. Rep. 2011; 1: 1-5
        • Gorden E.M.
        • Sturk-Andreaggi K.
        • Marshall C.
        Capture enrichment and massively parallel sequencing for human identification.
        Forensic Sci. Int. Genet. 2021; 53102496
        • Rohland N.
        • Glocke I.
        • Aximu-Petri A.
        • Meyer M.
        Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing.
        Nat. Protoc. 2018; 13: 2447-2461
        • Loreille O.
        • Tillmar A.
        • Brandhagen M.D.
        • Otterstatter L.
        • Irwin J.A.
        Improved DNA extraction and illumina sequencing of DNA recovered from aged rootless hair shafts found in relics associated with the romanov family.
        Genes. 2022; 13: 202