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The population genetics characteristics of Ion AmpliSeq™ MH-74 plex microhaplotype research panel

  • Shengqiu Qu
    Correspondence
    Corresponding author at: Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.
    Affiliations
    Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China

    Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany
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  • Author Footnotes
    † We would like to dedicate this paper to Peter M. Schneider, who unfortunately passed away just before the paper was submitted for publication. He played an essential role in the research described here and he will be sincerely missed.
    Peter M. Schneider
    Footnotes
    † We would like to dedicate this paper to Peter M. Schneider, who unfortunately passed away just before the paper was submitted for publication. He played an essential role in the research described here and he will be sincerely missed.
    Affiliations
    Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany
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  • Robert Lagacé
    Affiliations
    Human Identification Division HID – MPS, Thermo Fisher Scientific, South San Francisco, USA
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  • Maximilian Neis
    Affiliations
    Institute of Legal Medicine, Faculty of Medicine, University of Cologne, Cologne, Germany
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  • Weibo Liang
    Affiliations
    Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
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  • Lin Zhang
    Affiliations
    Department of Forensic Genetics, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China
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  • Author Footnotes
    † We would like to dedicate this paper to Peter M. Schneider, who unfortunately passed away just before the paper was submitted for publication. He played an essential role in the research described here and he will be sincerely missed.
Published:October 25, 2022DOI:https://doi.org/10.1016/j.fsigss.2022.10.068

      Abstract

      A large number of microhaplotype markers have been investigated for various forensic purposes in recent years. In the present study, we have analysed population genetic data and sequencing performance of the Ion AmpliSeq™ MH-74 plex microhaplotype research panel. A total of 178 samples from 3 major biogeographic regions including 54 Africans, 61 Iraqis from the Middle East and 63 Germans were genotyped using this panel on the Ion S5™ MPS platform (Thermo Fisher Scientific). Then we obtained the allele frequencies, the effective number of alleles (Ae), and the forensic efficiency parameters of the 74 MHs. Finally, STRUCTURE analysis was used to evaluate and compare the biostatistical efficiency of the 74 MHs in the 3 major biogeographic regions. All results demonstrated that the Ion AmpliSeq™ MH-74 plex microhaplotype research panel is characterized by a reliable sequencing performance.

      Keywords

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