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Efficiency evaluation of the LT-DNA traces analysis modifications

Published:October 19, 2022DOI:https://doi.org/10.1016/j.fsigss.2022.10.042

      Abstract

      Analysis of genetic profiles obtained from low template DNA samples (LT DNA) can be challenging because of increased probability of stochastic amplification artifacts occurrence. According to the recommendations of international genetic societies the quality of the LT-DNA traces results can be improved by applying low copy number (LCN) methods. Another strategy which allows to obtain better results of the analysis of LT-DNA traces is replicate the amplification of the same DNA sample and create consensus, composite (virtual pool profile) or real pool profile. The aim of the research was to analyze and compare the efficiency of modifications used in the testing of LT-DNA samples. Obtained results indicate that implementation of these methods in laboratory practice may lead to improvement in the quality of reported data from LT-DNA traces in genetic analyzes conducted to assist the justice system.

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      References

        • Benschop C.
        • Haned H.
        • Sijen T.
        Consensus and pool profiles to assist in the analysis and interpretation of complex low template DNA mixtures.
        Int. J. Leg. Med. 2013; 127: 11-23
      1. SWGDAM, Guidelines for STR Enhanced Detection Methods, (2014). Available at: http://media.wix.com/ugd/4344b0_29feed748e3742a5a7112467cccec8dd.pdf.

        • Cowen S.
        • Debenham P.
        • Dixon A.
        • et al.
        An investigation of the robustness of the consensus method of interpreting low-template DNA profiles.
        Forensic Sci. Int. Genet. 2011; 5: 400-406
        • Gill P.
        • Gusmao L.
        • Haned H.
        • et al.
        DNA commission of the International Society of Forensic Genetics: recommendations on the evaluation of STR typing results that may include drop-out and/or drop-in using probabilistic methods.
        Forensic Sci. Int. Genet. 2012; 6: 679-688
        • Gill P P.
        • Haned H.
        • Bleka O.
        • et al.
        Genotyping and interpretation of STR-DNA: low-template, mixturesand database matches-twenty years of research and development.
        Forensic Sci. Int. Genet. 2015; 18: 100-117
        • Parys-Proszek A.
        • Marcińska M.
        • Branicki W.
        • et al.
        Examination of LT-DNA traces – literature overview and general recommendations of the Polish Speaking Working Group of the International Society for Forensic Genetics (ISFG-PL).
        Arch. Forensic Med. Criminol. 2020; 70: 103-123