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DNA extraction of burnt bone and teeth casework samples using bead-beating homogenization technique

  • P. Varrathyarom
    Affiliations
    Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Forensic Serology and DNA, King Chulalongkorn Memorial Hospital and Thai Red Cross Society, Bangkok, Thailand
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  • T. Sathirapatya
    Affiliations
    Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
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  • W. Worrapitirungsi
    Affiliations
    Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
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  • K. Vongpaisarnsin
    Correspondence
    Correspondence to: 1873 Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok 10300, Thailand.
    Affiliations
    Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Forensic Serology and DNA, King Chulalongkorn Memorial Hospital and Thai Red Cross Society, Bangkok, Thailand
    Search for articles by this author
Published:October 18, 2022DOI:https://doi.org/10.1016/j.fsigss.2022.10.019

      Abstract

      Sample disruption was a necessary step for DNA isolation. Bone and teeth were useful biological sources particular in human remains and advance decomposed bodies. The compact bone and teeth required several preparation steps prior to analyzing process. However, the methods in standard protocol were laborious and time consuming. An alternating pulverization, bead beating homogenizer, was purposed in its effectiveness for forensic casework. (1) Here, we applied this technique to the burnt cracked bone and tooth that recovered from house fire for forensic DNA analysis. After cleansed an external surface, the eight multidirectional motion tissue homogenizer, Precellys® evolution, was utilized to pulverize bone and tooth followed by a DNA extraction and amplification. For detection with a capillary electrophoresis, full profiles of autosomal STRs and Y-chromosomal STRs were recovered from tooth sample but the partial profile STR was demonstrated in bone sample. The new technique in bone homogenization was less time consuming (around 30 s), less exposure to chemical agents (no need of liquid nitrogen), high efficiency, with high-throughput productivity.

      Keywords

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