In 50 cases, more than 3 mismatches between participants were identified and these samples where not taken for further analysis. In 235 cases either a complete match of Y-STR profiles between alleged relatives (86.0%) or mismatches at no more than 3 loci (14.0%) were observed (Table 1
presents the degree of relatedness between participants of each comparison. For Yfiler®
in two cases information on the number of meioses was not available and these cases were removed from analysis. Because of this the number of comparisons listed in Table 2
for this marker system (117) is less than the total number of comparisons which were analysed from Table 1
When using Yfiler®
(n = 119) a complete match between participants was observed in 91.6% cases, 1 mismatch – in 7.6% cases, and 3 mismatches – in 0.8% cases. When using Yfiler®
Plus (n = 58) a complete match between participants was observed in 79.3% cases, 1 mismatch – in 20.7% cases, and 2 mismatches – in 3.4% cases. In cases analysed by COrDYS-Y (n = 58) a complete match between participants was observed in 81.0% cases, 1 mismatch – in 13.8% cases, 2 mismatches – in 3.5% cases, and 3 mismatches – in 1.7% cases. All mismatches detected during the present study were by one STR repeat with a single exception of locus DYS627 (Yfiler®
Plus) where a mismatch of two repeats was found in one case. This locus is a rapidly mutating Y-STR [
- Ballantyne K.N.
- Goedbloed M.
- Fang R.
- et al.
Mutability of Y-chromosomal microsatellites: rates, characteristics, molecular bases, and forensic implications.
]. Overall, mismatches were present at all loci studied with exception of DYS392, DYS437, DYS438, DYS447, DYS448, DYS481 and DYS533. Loci DYS449, DYS460, DYS518, DYS570, and YGATAH4 showed increased mutation frequency with the highest observed in DYS570 (2.56*10−2
). The lowest mutation frequency was found in loci DYS389I, DYS390, DYS393 and DYS439 (2.19*10−3