If you don't remember your password, you can reset it by entering your email address and clicking the Reset Password button. You will then receive an email that contains a secure link for resetting your password
If the address matches a valid account an email will be sent to __email__ with instructions for resetting your password
Twenty three autosomal STR markers were analyzed to define which are the most informative in forensic genetic cases in Ecuadorian population. In this study, we report genetic data from 1434 and 1800 unrelated individuals for SE33 and other 22 STRs, respectively. The parameters analyzed were: polymorphism information content (PIC), heterozygosity, power of discrimination and power of exclusion. SE33 was the best marker for forensic identification purposes because it showed the highest polymorphism information content (PIC) of 0.9334; heterozygosity of 0.9191; power of discrimination of 0.9969 and power of exclusion of 0.8345. Hence, we recommend its use in routine and complex paternity cases. After SE33, the subsequent most informative STR markers for Ecuadorian population were PENTA E, FGA, D1S1656 and D19S433, and the less informative markers were VWA, D22S1045, D2S441, TPOX and D3S1358.
SE33 has been incorporated into several STR kits due to it's high polymorphism, it́s power of discrimination and the fact that more than 140 alleles have been reported to date [
]. The aim of this study was to compare the forensic efficiency parameters of 23 different STR to assess their forensic application in Ecuadorian Population and also report the genetic data for locus SE33. [
The samples analyzed in this study for 22 STR autosomal markers (D3S1358, D1S1656, D2S441, D10S1248, D13S317, PENTA E, D16S539, D18S51, D2S1338, CSF1PO, PENTA D, TH01, VWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA and D22S1045) were 1800 unrelated Ecuadorian individuals, which information were previously reported in Gaviria et al. [
]. For SE33 analysis, DNA samples from 1434 unrelated Ecuadorian individuals were extracted from whole blood following FTA method (Whatman). PCR amplification was performed using AmpFLSTR NGM SElect PCR Amplification kit, according to manufacturer's instructions (Applied Biosystems).
The ABI PRISM 3130 Genetic Analyzer and Genemapper v 3.2 software (Applied Biosystems) were used to analyze PCR products and population genetic analyses were calculated using Arlequin Software v. 3.5.1.3 [
PowerStats A. computer program for the analysis of population statistics (1999). Free Program Distributed by the Authors over the Internet from http://www.promega.com/geneticidtools
Some forensic efficiency parameters of the 23 autosomal markers investigated in Ecuadorian population are shown and detailed in Fig 1. SE33 marker showed twice the number of alelles: 47 (Table S1), compared to the next most variable STR locus D18S51 with 24.
Fig. 1Heterozygosity, polymorphism information content, power of discrimination and power of exclusion of 23 autosomal markers in Ecuadorian population.
The range of heterozygosity varied between 0.9191 (SE33) and 0.5251 (VWA), the markers with higher values of Heterozygosity and PIC are SE33, PENTA E, FGA and D1S1656 and the less informative markers were VWA, D22S1045, D2S441 and TPOX. The power of exclusion ranged from 0.8345 (SE33) to 0.2104 (VWA) (Table 1).
Table 1Heterozygosity, polymorphism information content, power of discrimination and power of exclusion of 23 autosomal markers in Ecuadorian population.
The highest degree of heterozygosity, PIC, power of discrimination and power of exclusion in SE33 Locus were reported in other population analyses as U.S, Poland, Germany and Portugal [
]. Following SE33 (0.9969) as the best power of discrimination marker for Ecuadorian population are with 0.9829 (Penta E), 0.9680 (FGA) and 0.9679 (D1S1656).
4. Conclusion
In this study we demonstrate that SE33 is the best STR for forensic identification purposes because it showed the highest: polymorphism information content (PIC) of 0.9334; heterozygosity of 0.9191; power of discrimination of 0.9969 and power of exclusion of 0.8345. Hence, we recommend its use in routine and complex paternity cases. After SE33, the subsequent most informative STR markers for Ecuadorian population were PENTA E, FGA, D1S1656 and D19S433, and the less informative markers were VWA, D22S1045, D2S441, TPOX and D3S1358.
Conflict of interest
None.
Ethical standards
Experiments comply with the current Ecuadorian Laws.
Role of funding
This research had financial support by Laboratory of Molecular Genetic of Cruz Roja Ecuatoriana—Cruz Vital, Quito, Ecuador.
Acknowledgments
For Cruz Roja Ecuatoriana—Cruz Vital and all the Ecuadorian people who participated with their samples in this study.
Appendix A. Supplementary data
The following are Supplementary data to this article:
PowerStats A. computer program for the analysis of population statistics (1999). Free Program Distributed by the Authors over the Internet from http://www.promega.com/geneticidtools
30184-0&id=gr1.jpg){kind=link}