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Editorial Board
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iii
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Proceedings of the 24th International ISFG Congress
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vi
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DIP–STR: A new marker for resolving unbalanced DNA mixtures
03 October 2011
Abstract: The genetic characterization of unbalanced mixed stains remains an important area where improvement is imperative. Most cases of aggression, homicide and sexual assault produce biological tr...
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D. Hall,
V. Castella
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e1-e2
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UTI preventing DNA degradation of storing urinary samples for genotyping
03 October 2011
Abstract: In forensic practices, individual identification of urinary samples is necessary when sample switching or handling are suspected. DNA degradation with time elapsing and the low yield of extr...
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Suhua Zhang,
Shumin Zhao,
Ruxin Zhu,
Gong Zhang,
Chengtao Li
et al.
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e3-e4
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Collaborative EDNAP exercises on messenger RNA/DNA co-analysis for body fluid identification (blood, saliva, semen) and STR profiling
26 September 2011
Abstract: RNA profiling is a new method for the identification of forensically relevant biological stains, such as blood, saliva, semen, vaginal secretions, menstrual blood, sweat and skin. To demonst...
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C. Haas,
E. Hanson,
N. Morling,
J. Ballantyne
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e5-e6
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Preliminary results of mitochondrial DNA sequence variation in Jujuy population (Argentina)
26 September 2011
Abstract: Jujuy is a province of Argentina, placed in the extreme northwest of the country, close to the borders with Chile, Bolivia and the Salta province of Argentina. Different studies on South Ame...
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A.M. López-Parra,
J.E. Dipierri,
M.D. Marrodán,
B.A. Gómez,
C. Baeza,
J.L. Pacheco,
I.F. Bejarano,
E. Alfaro,
M. García,
M. Tirado,
D. Testillano,
E. Arroyo-Pardo,
D.B. Lomaglio,
M.S. Mesa
et al.
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e7-e8
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When the alleged father is a close relative of the real father: The utility of insertion/deletion polymorphisms
05 October 2011
Abstract: The occurrence of Mendelian incompatibilities between true father and child is not an uncommon situation. Thus, in paternity investigations, when only a few are observed, it is possible that...
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M. Magalhaes,
N. Pinto,
C. Gomes,
R. Pereira,
A. Amorim,
C. Alves,
L. Gusmão
et al.
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e9-e10
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Evaluation of GeneMapper®ID-X Mixture Analysis tool
04 October 2011
Abstract: We have evaluated the ‘Mixture Analysis’ tool in GeneMapper®ID-X v1.1 (Applied Biosystems) to interpret two-person DNA mixtures. This package depends upon the evaluation of two different par...
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O. Hansson,
P. Gill
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e11-e12
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Database sample size effect on minimum allele frequency estimation: Database comparison analysis of samples of 4652 and 560 individuals for 22 microsatellites in Colombian population
05 October 2011
Abstract: With the aim of studying the effect of the database size on the frequency estimations of minimum and rare alleles, a comparative analysis was undertaken between two different size samples fr...
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Tomás Restrepo,
Martha Martinez,
Oscar Palacio,
Yeny Posada,
Silvana Zapata,
Leonor Gusmão,
Adriana Ibarra
et al.
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e13-e14
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Comparison of Identifiler®, Identifiler Plus® and Minifiler® performance in an initial paternity testing study on old skeletal remains at the forensic and legal medicine area of the Government of Andorra (Pyrenees)
12 October 2011
Abstract: The problem oftentimes faced by forensic scientists when working with DNA extracted from exhumations and skeletal remains is either DNA degradation or DNA contamination. Various methods have...
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C. Barrot,
C. Moreno,
C. Sánchez,
M. Rodríguez,
M. Ortega,
E. Huguet,
M.V. Lareu,
M. Gené
et al.
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e15-e16
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The Forensic Genetics Laboratory of the University of Barcelona: The evolution of paternity testing cases over the past 35 years
13 October 2011
Abstract: Biological paternity research in Catalonia (north-eastern Spain) began in 1976 when Dr. Castillo, Dr. Gelabert and Dr. Huguet studied the first case at the Department of Forensic and Legal M...
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C. Barrot,
C. Sánchez,
S. Jiménez,
M. Ortega,
E. Huguet,
M. Gené
et al.
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e17-e18
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X-chromosomal haplotype frequencies of four linkage groups in a North African population
05 October 2011
Abstract: Twelve X-STR loci in four clusters of linked markers were typed in a sample of 97 Moroccan men from different ethnic groups (Arabs, Berbers and Sahrawi) using the Investigator Argus X-12 Kit...
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A. Picornell,
K. Bentayebi,
M. Bouabdeallah,
R. Aboukhalid,
D. Squalli,
J.A. Castro,
M.M. Ramon,
S. Amzazi
et al.
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e19-e20
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A loophole in Spanish law and unsolved ethical issues in paternity testing
13 October 2011
Abstract: Article 18 of the current Spanish Constitution (which came into force in 1978) defends all citizens’ right to privacy. Accordingly, the new Code of Ethics of the Spanish Medical Association ...
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C. Barrot,
C. Sánchez,
S. Jiménez,
M. Ortega,
M. Gené
et al.
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e21-e22
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Experiences and surprises with PowerPlex ESI 17 and AmpflSTR NGM SElect in routine casework
28 September 2011
Abstract: The introduction of five “new European standard loci” (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) in forensic DNA analysis called for the development of new Multiplex PCR kits. One of ...
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Franz Neuhuber,
Eva Klausriegler,
Bettina Dunkelmann,
Gabriele Kreindl,
Jan Cemper-Kiesslich
et al.
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e23-e24
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New validated analytical process for convicted offender samples submitted to the Canadian National DNA Data Bank
03 October 2011
Abstract: Optimization and validation of direct amplification protocols for both AmpFlSTR® Identifiler® Direct (IDD) and PowerPlex® 16 HS (PP16HS) on the high-throughput 3730 DNA Analyzer (Applied Bio...
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N. Laurin,
A. De Moors,
C.J. Frégeau
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e25-e26
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Inferring ethnicity from the X-chromosome ALU insertions: Data from Western Mediterranean human groups
26 September 2011
Abstract: We investigated 9 X-chromosome ALU insertions, suitable for forensic purposes, in 645 healthy unrelated Mediterranean individuals (male and female). Forensic statistical parameters for these...
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K. Bentayebi,
M.M. Ramon,
J.A. Castro,
A. Barbaro,
M. Bouabdeallah,
S. Amzazi,
A. Picornell
et al.
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e27-e28
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Competition for DNA binding sites from denim dyes using the Promega DNA IQ™ paramagnetic beads
26 September 2011
Abstract: The competition between DNA and other components often present in casework specimen lysates (e.g. denim dyes) for the binding sites on the DNA IQ™ magnetic beads was examined. It was determi...
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C.J. Frégeau,
A. De Moors
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e29-e30
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Validation of the fluorescence-based Sperm Hy-Liter™ kit as a means to standardize spermatozoa identification in sexual assault cases
26 September 2011
Abstract: To enhance searching and standardize the identification of spermatozoa in sexual assault exhibits, the Sperm Hy-Liter™ kit was evaluated and validated using a large number of sexual assault ...
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A. De Moors,
T. Georgalis,
G. Armstrong,
J. Modler,
C.J. Frégeau
et al.
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e31-e32
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Genetic polymorphisms of eight X-STR loci of Mentype Argus X-8 kit in Koreans
31 October 2011
Abstract: We have examined 45 Korean haplotype transfers in 41 families at eight X-linked STRs (DXS10135 and DXS8378 in linkage group 1, DXS7132 and DXS10074 in linkage group 2, HPRTB and DXS10101 in ...
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Seong Yeon Yoo,
Nam Soo Cho,
Sun Wha Park,
Jung Hee Hwang,
Je Nyun Kim,
Ki Wha Chung,
Myun Soo Han
et al.
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e33-e34
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Automated scoring of Sperm Hy-Liter™-stained spermatozoa by the MetaSystems Metafer image analysis software system in sexual assault specimens
28 September 2011
Abstract: The MetaSystems Metafer image analysis software system was purchased three years ago in the hope of developing a routine approach in the RCMP Forensic Laboratories to automate the scoring of...
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A. De Moors,
C.J. Frégeau
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e35-e36
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X-chromosome in Italy: A database of 29 STR markers
28 September 2011
Abstract: We collected published and unpublished data from 17 contributing groups participating in the GeFI (Italian Forensic Geneticists). The total number of typed subjects was 1114 males and 777 fe...
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S. Presciuttini,
C. Toni,
M. Alù,
A. Asmundo,
L. Baldassarri,
A. Barbaro,
L. Caenazzo,
E. Carnevali,
N. Cerri,
E. D’Aloia,
C. Di Nunzio,
V. Onofri,
G. Peloso,
S. Pelotti,
A. Piccinini,
C. Robino,
S. Turrina,
M. Venturi,
R. Domenici
et al.
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e37-e38
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Quality control of DNA from formalin fixed and paraffin wax embedded prostate biopsy for forensic analysis
28 September 2011
Abstract: In human identification laboratories the quality assurance in the harmonization of the thermocyclers and the standardization of the sensitivity tests, mainly for reduced and previously manip...
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V.D. Cantagalli,
K.R.M. Leite,
M. Srougi,
G.J.F. Gattás
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e39-e40
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The potential risk of formalin fixed and paraffin wax embedded prostate biopsies for human identification
28 September 2011
Abstract: The genetic instability in malignant tumors and the possible DNA degradation in archived histological samples are potential confounder factors that can interfere in the human identification ...
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G.J.F. Gattás,
V.D. Cantagalli,
K.R.M. Leite,
M. Srougi
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e41-e42
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Optimization and validation of the SNPforID 34-SNPplex for POP7™
28 September 2011
Abstract: The SNPforID 34-SNPplex is a multiplex of ancestry informative single nucleotide polymorphism markers and was originally designed for POP6™ polymer gels. Due to the higher viscosity of POP7™...
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Bram Bekaert,
Jurgen Wens,
Ronny Decorte
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e43-e44
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An investigation of the presence of DNA on unused laboratory gloves
12 October 2011
Abstract: The presence of background DNA in forensic laboratories is potentially a significant contamination risk to criminal investigations. As part of the laboratory's Environmental Monitoring (EM) ...
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Runa Daniel,
Roland A.H. van Oorschot
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e45-e46
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LMD-assisted single cell DNA typing of forensic biological evidence: Issues of the cell type and sample condition
26 September 2011
Abstract: The practice of DNA typing of single cells in forensic biological evidence may have peculiarities. For instance, some laser microdissected (LMD) single cell preparations fail to give any PCR...
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Sergey Leonov,
Elena Zemskova,
Pavel Ivanov
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e47-e48
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Investigation of Paternity Loss: is statistical evaluation reliable? A case report
05 October 2011
Abstract: We report the experience of a laboratory that was charged by a Court to conduct an analysis to establish the paternity of a woman: her mother was alive, the alleged father was deceased, but ...
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Nicoletta Cerri,
Luciana Caenazzo
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e49-e50
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Mitochondrial DNA-control region sequence variation in the NE Portuguese Jewish community
28 September 2011
Abstract: The cultural phenomenon of Crypto-Judaism, defined as the secret adherence to Judaism while publicly professing another faith, arose in Portugal in the beginning of the 16th century after th...
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J.C. Teixeira,
I. Nogueiro,
A. Goios,
L. Gusmão,
A. Amorim,
L. Alvarez
et al.
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e51-e52
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Population genetic data for 15 autosomal STR loci in four Venezuelan states (Aragua, Carabobo, Zulia and Táchira)
05 October 2011
Abstract: Fifteen autosomal DNA markers (D3S1358, TH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1P0, PENTA D, VWA, D8S1179, TPOX and FGA) were genotyped in a total of 1290 unrela...
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M. Figuera,
A. Dictamen,
C. Marrero,
L. Borjas,
R. Ferreira,
M. Álvarez
et al.
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e53-e54
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Using X-chromosomal markers in rape investigation
26 September 2011
Abstract: The X-chromosomal markers are increasingly used in forensic genetics, particularly for relationship testing. Their use has become a valuable tool in complex cases of kinship but rather in cr...
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M. Lancia,
S. Severini,
A. Coletti,
G. Margiotta,
M. Dobosz,
E. Carnevali
et al.
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e55-e56
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Colombian results of the interlaboratory Quality Control Exercise 2009–2010
05 October 2011
Abstract: Colombian Reference National Laboratory, GENES LTDA, have organized and coordinated for the past two years (2009 and 2010) the Quality Control Exercise for laboratories undertaking paternity...
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J.J. Builes,
D. Aguirre,
A. Manrique,
Y. Puerto,
M.L. Bravo,
A. Gaviria,
A. Gutierrez,
M. Muñoz,
D. Fonseca,
W. Usaquen,
A. Castillo,
C. Pineda,
N. Ugalde,
R.M.B. Cicarelli,
A. Ibarra,
D.M. Trejos,
L.D. Hudy,
M. De Castro,
L.F. Díaz,
D. Quiceno,
A. Pinzón,
M. Gavilan,
D. Sánchez,
M. Roa,
H. Ossa,
G. Iannacone,
L. Mendoza,
M. Ruiz,
L. Solis,
L. Pareja,
A. Guevara,
A. Carracedo,
L. Gusmão
et al.
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e57-e58
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Genetic data of 10 X-STR in a Colombian population of Bolivar Department
28 September 2011
Abstract: X chromosome markers have been used in human identification and paternity testing since the early 1970s. In recent years it has been evaluated the informative power of X-linked STRs (X-STRs)...
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B. Martínez,
J.J. Builes,
L. Gusmão,
M. Manrique,
D. Aguirre,
Y. Puerto,
L. Caraballo,
M.L. Bravo
et al.
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e59-e60
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Validation of BTA™ lysis buffer for DNA extraction from challenged forensic samples
07 October 2011
Abstract: Isolation of DNA from forensic samples is strongly conditioned by many factors such as wide variety of samples, degradation by environmental exposure, presence of PCR inhibitors or limited q...
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A. Barbaro,
P. Cormaci,
G. Falcone
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e61-e62
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Recovery of DNA from paraffin embedded tumour samples of pediatric sarcomas
04 October 2011
Abstract: The main challenge of DNA extraction from old paraffin embedded tissue sections is to obtain analyzable DNA. The aim of this work was to recover DNA from paraffin embedded tumour samples. We...
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M. Marino,
A. Mampel,
S. Furfuro,
M. Di María,
J. Oliva,
A.L. Vargas
et al.
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e63-e64
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Haplotype frequencies and mutation rates for 17 Y-STRs in a sample from Mendoza province (Argentina)
04 October 2011
Abstract: A sample of 566 unrelated males from Mendoza province was typed for 12 Y-STRs loci, 237 of these males were typed for 17 Y-STRs. When analyzing only the minimal haplotype of 9 Y-STRs we foun...
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Miguel Marino,
Sandra Furfuro
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e65-e66
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Validation of AmpFLSTR NGM SElect™ PCR amplification kit on forensic samples
07 October 2011
Abstract: The AmpFLSTR NGM SElect™ is a next generation kit developed by Applied Biosystems that contains the 5 new loci specified in the recently expanded European Standard Set (ESS) together with th...
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A. Barbaro,
P. Cormaci,
A. Agostino
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e67-e68
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DNA extraction using the QIAsymphony: Evaluation of PCR inhibitor removal
28 September 2011
Abstract: The goal of this study was to compare the quantity and purity of DNA extracted from biological traces using the QIAsymphony robot with that of the manual QIAamp DNA mini kit currently in use...
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V. Castella,
D. Kummer,
C. Gehrig,
D. Hall
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e69-e70
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Use of universal reporter primers in multiplex PCR of autosomal loci
26 September 2011
Abstract: At present forensic identification and parentage testing is invariably carried out using multiplex PCR amplification kits in which many loci, whether they be STRs, SNPs or DIPs are analyzed ...
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Elaine C. Favaro,
Denilce R. Sumita,
Martin R. Whittle
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e71-e72
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Use of matrix standards for new fluorophores in capillary sequencers
26 September 2011
Abstract: Most laboratories presently carrying out forensic identification and parentage testing use capillary DNA sequencers to separate and visualize the alleles that are produced by multiplex PCR a...
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Martin R. Whittle,
Elaine C. Favaro,
Carlos W. Francischini,
Denilce R. Sumita
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e73-e74
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SNPs in mitochondrial DNA coding region used to discriminate common sequences in HV1–HV2–HV3 region
28 September 2011
Abstract: Regions HV1, HV2 and HV3 of mitochondrial DNA (mtDNA) are routinely analyzed for forensic and evolutionary purposes due to the high polymorphic rate but sometimes show limited power of discr...
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C. Fridman,
M.M.S.G. Cardena,
E.A. Kanto,
M.B.C. Godinho,
F.T. Gonçalves
et al.
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e75-e76
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Evaluation of the relationship between mitochondrial haplogroup and development of heart failure in Brazilian sample
28 September 2011
Abstract: Recently, some studies have been suggested an association between mitochondrial haplogroups (mtDNA Hg) and pathogenesis of cardiovascular diseases, showing an influence of ethnic origin with...
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C. Fridman,
M.M.S.G. Cardena,
J.E. Krieger,
A.C. Pereira
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e77-e78
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Analysis of complex DNA mixtures using the Forensim package
28 September 2011
Abstract: We describe a new user-friendly module of the Forensim package of the R software, LRmix, which enables the interpretation of complex STR profiles involving multiple replicates, multiple cont...
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Hinda Haned,
Peter Gill
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e79-e80
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Allele frequencies of 15 STRs in a representative sample of Entre Ríos province of Argentina population
13 October 2011
Abstract: Allele frequencies for 15 short tandem repeat (STR) loci present in AmpFℓSTR® Identifiler® PCR Amplification Kit (Applied Biosystems) were obtained from a sample of 839 unrelated individuals...
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G.G. Martinez,
L.C. Schaller,
A. Brondani,
M. Bolea,
B. Martinez-Jarreta
et al.
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e81-e82
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New alternative for human identification. Investigator IDplex Kit – QIAGEN® reproducibility: Latin American interlaboratory study
07 October 2011
Abstract: In 2010 QIAGEN® launched to eight kits of different combinations of STRs, including the Investigator IDplex Kit. This kit allows amplification in one PCR 16 markers. The aim of this study wa...
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J.J. Builes,
D. Trejos,
D. Suárez,
S. Moreno,
L. Siza,
M. Acevedo,
A. Castillo,
L. Beltrán,
A. Ibarra,
R.M.B. Cicarelli,
A. Gaviria,
L. Zuluaga
et al.
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e83-e84
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Concordance testing with the new STR kits and the influence on the Swiss National DNA database
26 September 2011
Abstract: The European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling Group (EDNAP) recommended the extension of the European Standard Set by 5 additional loci regarding...
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D. Dion,
A. Sulzer,
A. Kratzer
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e85-e86
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A primer binding site mutation at the D2S1338 locus resulting in a loss of amplification
13 October 2011
Abstract: An apparent paternal genetic incompatibility at the D2S1338 locus due to false homozygosity is described. It was disclosed after amplification with the AmpFlSTR® Identifiler® PCR Amplificati...
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E.M. Dauber,
B. Glock,
W.R. Mayr
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e87-e88
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Internal validation of Tecan robots (Freedom EVO® 150 and 75) for PCR and capillary electrophoresis setup
26 September 2011
Abstract: We validated and established an automated method for PCR and capillary electrophoresis setup (CE setup), using the Tecan Freedom EVO® 150 and 75 automated liquid handling workstations powere...
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Nadja V. Morf,
Andrea Sulzer,
Adelgunde Kratzer,
Walter Bär
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e89-e90
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The Pinpoint DNA Isolation System as a novel DNA sampling method in forensic biology
28 September 2011
Abstract: The Pinpoint DNA Isolation System (Zymo Research) uses a dissolved polymer compound to remove and extract DNA from slide mounted pathology specimens. This polymer is applied to a non-porous ...
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Timothy J. Verdon,
Kaye N. Ballantyne,
R. John Mitchell,
Roland A.H. van Oorschot
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e91-e92
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Evaluating the efficiency of DNA extraction methods from different substrates
28 September 2011
Abstract: The efficiency of extracting DNA directly from substrates varies according to a range of factors including the type of substrate and the extraction technique. Two routinely used DNA extracti...
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Timothy J. Verdon,
R. John Mitchell,
Roland A.H. van Oorschot
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e93-e94
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Reliability of results in sibship cases
03 October 2011
Abstract: Genetic exclusion of siblinghood of two persons, without parental findings, is basically not possible. Both the positive and the negative proof of descent can only be provided by statistics....
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A. Sulzer,
D. Dion,
A. Kratzer
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e95-e96
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Genetic diversity of 17 Y-chromosomal short tandem repeat loci in northeast Germany
28 September 2011
Abstract: The 17 short tandem repeat loci included in the Y-filer™ PCR amplification kit from Applied Biosystems (AB) DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, ...
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Eileen Boljahn,
Sylvio Tetzlaff,
Andreas Buettner,
Iris Lindner
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e97-e98
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SNP typing of crime case samples with the SNPforID multiplex assay
26 September 2011
Abstract: The SNPforID multiplex was tested on typical crime case samples. In a mixture study, both controlled experiments with samples mixed in known ratios and a blind study were conducted. All mixt...
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C. Børsting,
B.B. Hjort,
N. Morling
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e99-e100
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Genetic data for the locus SE33 in a Southern Italy population with AmpFlSTR NGM SElect™ PCR amplification kit
07 October 2011
Abstract: A genetic population study for SE33 locus has performed from a sample of 130 individuals coming from South of Italy (Calabria) since at least 3 generations, using the next generation multipl...
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A. Barbaro,
P. Cormaci,
A. La Marca
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e101-e102
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Direct PCR by the AmpFlSTR NGM™ kit for database purpose
07 October 2011
Abstract: To fully maximize the utility of DNA databases for tracing the origins of criminal activity, the streamlining of database sample processing must also be accompanied by high performance chemi...
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A. Barbaro,
P. Cormaci,
S. Votano
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e103-e104
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The potential forensic utility of two single nucleotide polymorphisms in predicting biogeographical ancestry
28 September 2011
Abstract: DNA-based prediction of an individual biogeographical ancestry would provide some investigative lead value in specific forensic cases. Recent studies have shown that single nucleotide polymo...
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Yan Gu,
Libing Yun,
Lushun Zhang,
Fan Yang,
Yiping Hou
et al.
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e105-e106
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Analysis of tri-allelic SNPs for forensic purpose in Chinese Han population
26 September 2011
Abstract: Single nucleotide polymorphisms (SNPs) have been proved to be effective forensic marker for supplementary paternity testing and forensic identification due to their low mutation rate and dep...
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L. Zha,
L.B. Yun,
H.B. Luo,
J. Yan,
Y.P. Hou
et al.
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e107-e108
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Statistical evaluation of pre-laboratory and laboratory factors that influence DNA recovery from archaeological material
28 September 2011
Abstract: We evaluated the influence of different factors over the efficiency of DNA amplification and PCR inhibition from 89 archaeological samples. We studied the effect of sample age and location t...
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Cristina Gamba,
Eva Fernández,
Ana María López-Parra,
Eduardo Arroyo-Pardo
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e109-e110
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Oral swab, as simple as that? A case report
28 September 2011
Abstract: Although performing oral swabs is a very simple, fast and cheap procedure, it should always be kept in mind the importance of this step in DNA typing. We report a case of voluntary contamina...
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A. Verzeletti,
N. Cerri,
V. Cortellini,
F. De Ferrari
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e111-e112
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Evaluation of PowerPlex ESI 17® amplification kit in an admixed Hispano-Amerindian population sample of Valparaíso, Chile
28 September 2011
Abstract: The PowerPlex ESI 17® (PP17) amplification kit was designed for European population (ENFSI and EDNAP recommendations). Routinely, DNA testing in our population was performed using the AmpFℓS...
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Manríquez Jose,
Rojas Sergio,
Yáñez Maria Oriana,
Molina Graciela
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e113-e114
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Analysis of severely compromised skeletal remains by the use of the latest generation kits
28 September 2011
Abstract: In recent years, European working groups (ENFSI-EDNAP) have strongly encouraged the development of new amplification kits that allow to obtain DNA profiles even on difficult samples (degrade...
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S. Severini,
M. Lancia,
S. Massetti,
A. Coletti,
L. Carlini,
E. Carnevali
et al.
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e115-e116
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