Analysis of AmpFlSTR® MiniFiler™ loci and its forensic application

Nakamura, Shigeki; Murakami, Chikako; Maeda, Kazuho; Kobayashi, Masamune; Irie, Wataru; Wada, Bunta; Hayashi, Maiko; Sasaki, Chizuko; Furukawa, Masataka; Kurihara, Katsuyoshi

doi:10.1016/j.fsigss.2009.08.196

« Previous Next »Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 19-20, December 2009

Analysis of AmpFlSTR^® MiniFiler™ loci and its forensic application

Department of Legal Medicine, Kitasato University School of Medicine, Sagamihara 228-8555, Japan

Received 14 August 2009; accepted 14 August 2009. published online 15 October 2009.

Abstract

The allele frequencies of eight MiniFiler™ loci have been analyzed in 101 Japanese individuals living in Kanagawa with informed consent by means of ABI 310 Genetic Analyzer. A total of 7 alleles for D13S317, 8 alleles for D7S820, 11 alleles for D2S1338, 11 alleles for D21S11, 5 alleles for D16S539, 14 alleles for D18S51, 8 alleles for CSF1PO, and 13 alleles for FGA were observed. The polymorphic profiles of these MiniFiler™ loci in the present study were essentially the same as those obtained by using the AmpFlSTR^® Identifiler^® PCR Amplification kit. The combined matching probability of eight MiniFiler™ loci and cumulative probability of paternity exclusion were estimated as 1.97×10⁻¹⁰ and 0.9996, respectively. The MiniFiler™ kit was useful for individual identification in forensic analysis.