Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 27-28, December 2009

Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit for forensic STR analysis

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, 2100 Copenhagen, Denmark

Received 1 September 2009; accepted 1 September 2009. published online 12 October 2009.

Abstract 

Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR® SGM Plus™ kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.

Keywords: Short tandem repeats (STRs), AmpFℓSTR® SEfiler Plus™ PCR Amplification kit, Forensic genetics

 

PII: S1875-1768(09)00222-4

doi:10.1016/j.fsigss.2009.09.014

Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 27-28, December 2009

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