Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit for forensic STR analysis
Received 1 September 2009; accepted 1 September 2009. published online 12 October 2009.
Abstract
Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR® SGM Plus™ kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.
Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, 11 Frederik V's Vej, 2100 Copenhagen, Denmark
ABSTRACT