Volume 2, Issue 1 , Pages 27-28, December 2009
Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit for forensic STR analysis
Abstract
Validation of the AmpFℓSTR® SEfiler Plus™ PCR Amplification kit with 29 and 30 PCR cycles for forensic STR analysis demonstrated that the kit had fewer artefacts than the AmpFℓSTR® SGM Plus™ kit (28 PCR cycles). The SEfiler Plus kit was more sensitive and devoid of colour artefacts, but showed more stutters, drop-ins, drop-outs and allelic imbalances.
Keywords: Short tandem repeats (STRs), AmpFℓSTR® SEfiler Plus™ PCR Amplification kit, Forensic genetics
PII: S1875-1768(09)00222-4
doi:10.1016/j.fsigss.2009.09.014
© 2009 Elsevier Ireland Ltd. All rights reserved.
Volume 2, Issue 1 , Pages 27-28, December 2009
