Volume 2, Issue 1 , Pages 25-26, December 2009
Development and validation of a next generation STR ESS-pentaplex
Abstract
We constructed a simple STR pentaplex of new loci recommended as next generation markers for the European Standard Set (ESS) comprising normal-amplicon STRs: D12S391 and D1S1656, plus mini-amplicon STRs: D2S441, D10S1248 and D22S1045. Validation of the pentaplex included evaluation of its ability to amplify DNA from a variety of degraded forensic casework samples. Although the ESS-pentaplex was designed in the first instance to generate allele frequency data to supplement existing databases of established STRs, the multiplex proved to be a valuable tool for the analysis of challenging DNA when certain markers of Identifiler or MiniFiler occasionally failed.
Keywords: Short tandem repeat, STR, Human identification, Multiplex PCR, Degraded DNA
PII: S1875-1768(09)00203-0
doi:10.1016/j.fsigss.2009.08.190
© 2009 Elsevier Ireland Ltd. All rights reserved.
Volume 2, Issue 1 , Pages 25-26, December 2009
