Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 23-24, December 2009

The single most polymorphic STR Locus: SE33 performance in U.S. populations

  • John M. Butler

      Affiliations

    • National Institute of Standards and Technology, Gaithersburg, MD 20899-8312, USA
    • Corresponding Author InformationCorresponding author.
    • ,
  • Carolyn R. Hill

      Affiliations

    • National Institute of Standards and Technology, Gaithersburg, MD 20899-8312, USA
    • ,
  • Margaret C. Kline

      Affiliations

    • National Institute of Standards and Technology, Gaithersburg, MD 20899-8312, USA
    • ,
  • David L. Duewer

      Affiliations

    • National Institute of Standards and Technology, Gaithersburg, MD 20899-8312, USA
    • ,
  • Cynthia J. Sprecher

      Affiliations

    • Promega Corporation, Madison, WI 53711, USA
    • ,
  • Robert S. McLaren

      Affiliations

    • Promega Corporation, Madison, WI 53711, USA
    • ,
  • Dawn R. Rabbach

      Affiliations

    • Promega Corporation, Madison, WI 53711, USA
    • ,
  • Benjamin E. Krenke

      Affiliations

    • Promega Corporation, Madison, WI 53711, USA
    • ,
  • Douglas R. Storts

      Affiliations

    • Promega Corporation, Madison, WI 53711, USA

Received 25 August 2009; accepted 27 August 2009. published online 15 October 2009.

Abstract 

The STR locus SE33 (ACTBP2) located on chromosome 6 (6q14) is arguably the most polymorphic marker examined thus far by the forensic community with a heterozygosity of >0.95 in some populations. Three different primer sets were utilized in this study in order to assess the possibilities of primer binding site mutations. Population variation was measured in 460 U.S. Caucasian, 445 African American, 336 Hispanic, and 202 Asian samples along with mutation rates from almost 400 father–son pairs. In addition, the 10 genomic DNA components in NIST Standard Reference Material SRM 2391b were sequenced and found to exhibit a variety of additional base changes, insertions, and deletions outside of the SE33 repeat region.

Keywords: DNA, Forensic DNA, STR, SE33, ACTBP2, Population data, Concordance study, Mutation rate

 

PII: S1875-1768(09)00186-3

doi:10.1016/j.fsigss.2009.08.173

Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 23-24, December 2009

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