Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 21-22, December 2009

Development of two new Mini-STR multiplex assay for typing archival Bouin's fluid-fixed paraffin-embedded tissues

  • Stefania Turrina

      Affiliations

    • Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi – P.le L.A. Scuro, 37134 Verona, Italy
    • Corresponding Author InformationCorresponding author. Tel.: +39 045 8124942; fax: +39 045 8027479.
    • ,
  • Giulia Filippini

      Affiliations

    • Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi – P.le L.A. Scuro, 37134 Verona, Italy
    • ,
  • Luciana Caenazzo

      Affiliations

    • Department of Environmental Medicine and Public Health, Legal Medicine Section, University of Padua, Via Falloppio 50, 35121 Padova, Italy
    • ,
  • Domenico De Leo

      Affiliations

    • Department of Medicine and Public Health, Institute of Legal Medicine, Forensic Genetic Laboratory, University of Verona, Policlinico G.B. Rossi – P.le L.A. Scuro, 37134 Verona, Italy

Received 21 August 2009; accepted 26 August 2009. published online 05 October 2009.

Abstract 

Short amplicon autosomal short tandem repeat (Mini-STR) assay has proved to be a highly useful tool in forensic applications, especially for highly degraded DNA samples that typically result in partial profiles and total loss of information from regular STR amplicons.

In this study two new quadruplex systems were designed to get nuclear DNA profile from degraded forensic casework samples. In order to obtain PCR products less than 120bp in size, primer pairs of eight STR markers, included in available commercially multiplex PCR kits, were redesigned and assembled in two PCR-multiplexes: D8S1179, D3S1358, TPOX, D16S539 and CSF1P0, TH01, D13S317, D5S818.

After validation, these two Mini-STR quadruplex were employed in paternity testing case that involved DNA extraction from archival postmortem Bouin's fluid-fixed paraffin-embedded tissue where commercial kit yielded low success. The results obtained with the present Mini-STR PCR-multiplexes proved clearly demonstrating their usefulness in analyzing degraded DNA samples.

Keywords: Autosomal Mini-STR, Quadruplex, Degraded DNA

 

PII: S1875-1768(09)00169-3

doi:10.1016/j.fsigss.2009.08.156

Forensic Science International: Genetics Supplement Series
Volume 2, Issue 1 , Pages 21-22, December 2009

Article Tools