Automated washing of FTA Card punches and PCR setup for reference samples using a LIMS-controlled Sias Xantus automated liquid handler

Puncher outputfile. The outputfile contained among other information the name of the punching template used (IDFL_FTA), name of the batch (2009-0615_IDFL_P_HPE), well number and corresponding parent sample number. The sample with sample number “010355” in well D01 was not in queue for the IDFL test.

(A) Screendump from the LabWare LIMS displaying the batch layout. The batch view displays a view of the child samples (sample fractions) being processed. Hence, sample numbers were child sample numbers. Position 4 contained the sample automatically removed by the LabWare LIMS. Re-runs are indicated with either “DE” or “F”. (B) Worklist for the Sias Xantus automated liquid handler. The subsequent PCR batch was controlled by a worklist generated by the LabWare LIMS. Position 4 (well D4) contained the affected sample excluded from the batch. The “0” indicates wells where PCR master mix was not dispensed while “1” indicates wells into which PCR master mix was dispensed. The “TwintecPrefill_001” signifies the microtitreplate number 1.