Automated extraction of DNA and PCR setup using a Tecan Freedom EVO® liquid handler

Received 8 July 2009; accepted 9 July 2009. published online 01 September 2009.

Abstract

We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO® liquid handler mounted with a Te-MagS™ magnetic separation device. The DNA was extracted using the Qiagen MagAttract® DNA Mini M48 kit. The DNA was amplified using AmpFℓSTR® Identifiler®, Y-filer® (Applied Biosystems), GenePrint® FFFL and PowerPlex® Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025.

Keywords: Laboratory automation, Nucleic acid extraction, Magnetic beads, Reference samples

Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health Sciences, University of Copenhagen, Denmark

Corresponding author. Tel.: +45 2875 6311.

PII: S1875-1768(09)00002-X

doi:10.1016/j.fsigss.2009.07.002

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