Use of “AnyDirect PCR buffer” for PCR amplification of washed bloodstains: A case report

Received 2 September 2007; accepted 10 October 2007.

Abstract

AnyDirect PCR buffer (BioQuest) permits to perform direct PCR from different kinds of forensic samples (blood, saliva, sperm, etc.) without any DNA purification step.

This is very useful in particular when working with small quantity of sample since it avoids the risk of loosing sample step by step as often happens with traditional DNA extraction procedures.

In the present casework we analyzed some bloodstains found by luminol test onto washed clothes and linen: all samples were analyzed either using the AmpFlSTRs Identifiler kit (Applied Biosystems).

Keywords: Washed bloodstains, Direct PCR, MiniSTRs

a Department of Forensic Genetics, SIMEF-89128 Reggio Calabria, Italy

b Department of Chemistry, SIMEF-89128 Reggio Calabria, Italy

c SIMEF Director-89128 Reggio Calabria, Italy

Corresponding author. Tel.: +39 0965891184; fax: +39 0965891125.

PII: S1875-1768(08)00230-8

doi:10.1016/j.fsigss.2007.10.201

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