Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 13-15 , August 2008

A multiplexed system for quantification of human DNA and human male DNA and detection of PCR inhibitors in biological samples

Received 26 September 2007 ,Accepted 11 October 2007.

References 

  1. Green RL, et al. Developmental validation of the Quantifiler™ real-time PCR kits for the quantification of human nuclear samples. J. Forensic Sci. 2005;50:809–825
  2. Nicklas JA, Buel E. Simultaneous determination of total human and male DNA using a duplex real-time PCR assay. J. Forensic Sci. 2006;51:1005–1015
  3. Swango KL, et al. Developmental validation of a multiplex qPCR assay for assessing the quantity and quality of nuclear DNA in forensic samples. Forensic Sci. Int. 2006;170:35–45
  4. Walker JA, et al. Multiplex polymerase chain reaction for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA: application in human identification. Anal. Biochem. 2005;337:89–97
  5. Furtado MR, et al. Real-time quantitative PCR in the analysis of gene expression. In:  Demidov VV,  Broude N editor. DNA Amplification: Current Technologies and Applications. Norfolk, UK: Horizon Bioscience; 2004;p. 131–146

PII: S1875-1768(08)00189-3

doi: 10.1016/j.fsigss.2007.10.197

Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 13-15 , August 2008