Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 19-21 , August 2008

Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

  • Paul Brotherton

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • Henry Wellcome Ancient Biomolecules Centre, University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    • Corresponding Author InformationCorresponding author. Tel.: +441457858605.
    • ,
  • Phillip Endicott

      Affiliations

    • Henry Wellcome Ancient Biomolecules Centre, University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    • ,
  • Mark Beaumont

      Affiliations

    • School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AG, UK
    • ,
  • Ross Barnett

      Affiliations

    • Department of Archaeology, University of Durham, South Road, Durham DH1 3L, UK
    • ,
  • Jeremy Austin

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • ,
  • Alan Cooper

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • ,
  • Juan J. Sanchez

      Affiliations

    • National Institute of Toxicology and Forensic Sciences, Canary Islands delegation, 38320, Tenerife, Spain

Received 20 August 2007 ,Accepted 9 October 2007.

References 

  1. Brotherton P, Endicott P, Sanchez JJ, Beaumont M, Barnett R, Austin J, et al. Novel high-resolution characterization of ancient DNA reveals C>U-type base modification events as the sole cause of post mortem miscoding lesions. Nucl. Acid. Res. 2007;
  2. Gilbert MT, Hansen AJ, Willerslev E, Rudbeck L, Barnes I, Lynnerup N, et al. Characterization of genetic miscoding lesions caused by postmortem damage. Am. J. Hum. Genet. 2003;72:48–61
  3. Stiller M, Green RE, Ronan M, Simons JF, Du L, He W, et al Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA. Proc. Natl. Acad. Sci. USA. 2006;103:13578–13584

PII: S1875-1768(08)00152-2

doi: 10.1016/j.fsigss.2007.10.111

Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 19-21 , August 2008

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