Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 19-21, August 2008

Single primer extension (SPEX) amplification to accurately genotype highly damaged DNA templates

  • Paul Brotherton

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • Henry Wellcome Ancient Biomolecules Centre, University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    • Corresponding Author InformationCorresponding author. Tel.: +441457858605.
    • ,
  • Phillip Endicott

      Affiliations

    • Henry Wellcome Ancient Biomolecules Centre, University of Oxford, Department of Zoology, South Parks Road, Oxford OX1 3PS, UK
    • ,
  • Mark Beaumont

      Affiliations

    • School of Animal and Microbial Sciences, University of Reading, Reading RG6 6AG, UK
    • ,
  • Ross Barnett

      Affiliations

    • Department of Archaeology, University of Durham, South Road, Durham DH1 3L, UK
    • ,
  • Jeremy Austin

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • ,
  • Alan Cooper

      Affiliations

    • Australian Centre for Ancient DNA, School of Earth and Environmental Sciences, University of Adelaide, Adelaide, SA 5005, Australia
    • ,
  • Juan J. Sanchez

      Affiliations

    • National Institute of Toxicology and Forensic Sciences, Canary Islands delegation, 38320, Tenerife, Spain

Received 20 August 2007; accepted 9 October 2007.

Abstract 

High levels of non-authentic sequence data can be generated by traditional PCR-based methodologies when DNA is damaged, template numbers are small and/or the target amplification size too large. We therefore present an alternate methodology based on single primer extension (SPEX) amplification; that places no pre-defined size constraints on amplification and interacts with only one of the DNA strands at the target locus.

Keywords: DNA damage, Genotyping, Low copy number DNA, SNPs

 

PII: S1875-1768(08)00152-2

doi:10.1016/j.fsigss.2007.10.111

Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 19-21, August 2008

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