A more efficient extraction method of human bone resulting in improved DNA profiling
Received 20 August 2007; accepted 10 October 2007.
Abstract
Eight human bone samples, from a forensic case, were extracted in parallel using our standard protocol with and without PTB in the buffer. Both methods were sometimes inadequate for (complete) STR profiling, while the presence of PTB even decreases the DNA yield.
The complete decalcification of the bone extraction residues in an EDTA-solution with SDS recovered sufficient amounts of DNA, which resulted in complete STR profiling for all samples. Complete decalcification without SDS yielded even higher amounts of DNA and also complete STR profiling for all samples.
Similar results were obtained for the DNA extraction from a human tooth.
ABSTRACT