Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 4-6 , August 2008

Locked nucleic acids: Increased trace DNA amplification success with improved primers

  • K.N. Ballantyne

      Affiliations

    • Victoria Police Forensic Services Department, Macleod 3085, Victoria, Australia
    • Genetics Department, La Trobe University, Melbourne 3086, Victoria, Australia
    • Corresponding Author InformationCorresponding author at: 31 Forensic Drive, Macleod 3085, Victoria, Australia. Tel.: +61 3 9450 3519; fax: +61 3 9450 3601.
    • ,
  • R.A.H. van Oorschot

      Affiliations

    • Victoria Police Forensic Services Department, Macleod 3085, Victoria, Australia
    • ,
  • R.J. Mitchell

      Affiliations

    • Genetics Department, La Trobe University, Melbourne 3086, Victoria, Australia

Received 14 August 2007 ,Accepted 7 October 2007.

References 

  1. Reynisson E, Josefsen MH, Krause M, Hoorfar J. Evaluation of probe chemistries and platforms to improve the detection limit of real-time PCR. J. Microbiol. Methods. 2006;66:206–216
  2. Ugozzoli LA, Latorra D, Pucket R, Arar K, Hamby K. Real-time genotyping with oligonucleotide probes containing locked nucleic acids. Anal. Biochem. 2004;324:143–152
  3. Levin JD, Fiala D, Samala MF, Kahn JD, Peterson RJ. Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers. Nucleic Acids Res. 2006;34:e142
  4. Butler JM, Shen Y, McCord BR. The development of reduced size STR amplicons as tools for analysis of degraded DNA. J. Forensic Sci. 2003;48:1054–1064
  5. Latorra D, Arar K, Hurley JM. Design considerations and effects of LNA in PCR primers. Mol. Cell Probes. 2003;17:253–259
  6. Cobb BD, Clarkson JM. A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Res. 1994;22:3801–3805

PII: S1875-1768(08)00083-8

doi: 10.1016/j.fsigss.2007.10.052

Forensic Science International: Genetics Supplement Series
Volume 1, Issue 1 , Pages 4-6 , August 2008

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